Genetic characterization of simian immunodeficiency virus isolated from an African mandrill
Identifieur interne : 001479 ( Main/Exploration ); précédent : 001478; suivant : 001480Genetic characterization of simian immunodeficiency virus isolated from an African mandrill
Auteurs : H. Sakai [Japon] ; J. I. Sakuragi [Japon] ; S. Sakuragi [Japon] ; R. Shibata [Japon] ; M. Hayami [Japon] ; A. Ishimoto [Japon] ; A. Adachi [Japon]Source :
- Archives of Virology [ 0304-8608 ] ; 1992-03-01.
English descriptors
- KwdEn :
- Acid residues, Adachi, African mandrill, Aids virus, Amino, Amino acids, Assay, Cell lysates, Cells transfected, Clone, Coding exon, Deletion, Functional domains, Gene mutants, Genetic characterization, Genome, Hayami, Human immunodeficiency virus type, Immunodeficiency, Infectious clone, Lentiviruses, Molecular clones, Mutant, Negative control, Primate, Primate immunodeficiency viruses, Primate lentiviruses, Proc natl acad, Progeny, Progeny virions, Proviral, Retrovirus, Sakai, Sakai etal, Shibata, Simian, Simian immunodeficiency virus, Sivmnd, Transactivation, Transactivation potentials, Transfected, Transfection, Viral, Virol, Virus, Wild type, Wild type level.
- Teeft :
- Acid residues, Adachi, African mandrill, Aids virus, Amino, Amino acids, Assay, Cell lysates, Cells transfected, Clone, Coding exon, Deletion, Functional domains, Gene mutants, Genetic characterization, Genome, Hayami, Human immunodeficiency virus type, Immunodeficiency, Infectious clone, Lentiviruses, Molecular clones, Mutant, Negative control, Primate, Primate immunodeficiency viruses, Primate lentiviruses, Proc natl acad, Progeny, Progeny virions, Proviral, Retrovirus, Sakai, Sakai etal, Shibata, Simian, Simian immunodeficiency virus, Sivmnd, Transactivation, Transactivation potentials, Transfected, Transfection, Viral, Virol, Virus, Wild type, Wild type level.
Abstract
Summary: We constructed an infectious molecular clone of simian immunodeficiency virus from an African mandrill (SIVMND). Upon transfection, this clone directed the production of progeny virus particles infectious to and cytopathic for CD4+ human leukemia cells. Thirteen frameshift proviral mutants with an alteration in the eight open reading frames of SIVMND were generated by recombinant DNA techniques, and were analyzed biologically and biochemically. While mutations in the structural genesgag, pol, andenv abolished viral growth and induction of cytopathology, mutants of thevif, vpr, andnef genes were fully biologically active. Of thetat andrev mutants, only onerev mutant grew in CD4+ cells with delayed kinetics. In reporter-based transient expression systems, transactivation potentials of thetat andrev mutants were evaluated. A mutant lacking 2nd coding exon oftat gene exhibitedtat activity similar to that of the wild type clone. The infectiousrev mutant was partially defective forrev gene activity.
Url:
DOI: 10.1007/BF01309624
Affiliations:
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Hayami</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Virus Research, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Ishimoto, A" sort="Ishimoto, A" uniqKey="Ishimoto A" first="A." last="Ishimoto">A. Ishimoto</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Virus Research, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author><author><name sortKey="Adachi, A" sort="Adachi, A" uniqKey="Adachi A" first="A." last="Adachi">A. Adachi</name><affiliation wicri:level="4"><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Virus Research, Kyoto University, Kyoto</wicri:regionArea><orgName type="university">Université de Kyoto</orgName><placeName><settlement type="city">Kyoto</settlement><region type="prefecture">Région du Kansai</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Archives of Virology</title><title level="j" type="abbrev">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><publisher>Springer-Verlag</publisher><pubPlace>Vienna</pubPlace><date type="published" when="1992-03-01">1992-03-01</date><biblScope unit="volume">125</biblScope><biblScope unit="issue">1-4</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="14">14</biblScope></imprint><idno type="ISSN">0304-8608</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acid residues</term><term>Adachi</term><term>African mandrill</term><term>Aids virus</term><term>Amino</term><term>Amino acids</term><term>Assay</term><term>Cell lysates</term><term>Cells transfected</term><term>Clone</term><term>Coding exon</term><term>Deletion</term><term>Functional domains</term><term>Gene mutants</term><term>Genetic characterization</term><term>Genome</term><term>Hayami</term><term>Human immunodeficiency virus type</term><term>Immunodeficiency</term><term>Infectious clone</term><term>Lentiviruses</term><term>Molecular clones</term><term>Mutant</term><term>Negative control</term><term>Primate</term><term>Primate immunodeficiency viruses</term><term>Primate lentiviruses</term><term>Proc natl acad</term><term>Progeny</term><term>Progeny virions</term><term>Proviral</term><term>Retrovirus</term><term>Sakai</term><term>Sakai etal</term><term>Shibata</term><term>Simian</term><term>Simian immunodeficiency virus</term><term>Sivmnd</term><term>Transactivation</term><term>Transactivation potentials</term><term>Transfected</term><term>Transfection</term><term>Viral</term><term>Virol</term><term>Virus</term><term>Wild type</term><term>Wild type level</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Acid residues</term><term>Adachi</term><term>African mandrill</term><term>Aids virus</term><term>Amino</term><term>Amino acids</term><term>Assay</term><term>Cell lysates</term><term>Cells transfected</term><term>Clone</term><term>Coding exon</term><term>Deletion</term><term>Functional domains</term><term>Gene mutants</term><term>Genetic characterization</term><term>Genome</term><term>Hayami</term><term>Human immunodeficiency virus type</term><term>Immunodeficiency</term><term>Infectious clone</term><term>Lentiviruses</term><term>Molecular clones</term><term>Mutant</term><term>Negative control</term><term>Primate</term><term>Primate immunodeficiency viruses</term><term>Primate lentiviruses</term><term>Proc natl acad</term><term>Progeny</term><term>Progeny virions</term><term>Proviral</term><term>Retrovirus</term><term>Sakai</term><term>Sakai etal</term><term>Shibata</term><term>Simian</term><term>Simian immunodeficiency virus</term><term>Sivmnd</term><term>Transactivation</term><term>Transactivation potentials</term><term>Transfected</term><term>Transfection</term><term>Viral</term><term>Virol</term><term>Virus</term><term>Wild type</term><term>Wild type level</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: We constructed an infectious molecular clone of simian immunodeficiency virus from an African mandrill (SIVMND). Upon transfection, this clone directed the production of progeny virus particles infectious to and cytopathic for CD4+ human leukemia cells. Thirteen frameshift proviral mutants with an alteration in the eight open reading frames of SIVMND were generated by recombinant DNA techniques, and were analyzed biologically and biochemically. While mutations in the structural genesgag, pol, andenv abolished viral growth and induction of cytopathology, mutants of thevif, vpr, andnef genes were fully biologically active. Of thetat andrev mutants, only onerev mutant grew in CD4+ cells with delayed kinetics. In reporter-based transient expression systems, transactivation potentials of thetat andrev mutants were evaluated. A mutant lacking 2nd coding exon oftat gene exhibitedtat activity similar to that of the wild type clone. The infectiousrev mutant was partially defective forrev gene activity.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région du Kansai</li></region><settlement><li>Kyoto</li></settlement><orgName><li>Université de Kyoto</li></orgName></list><tree><country name="Japon"><region name="Région du Kansai"><name sortKey="Sakai, H" sort="Sakai, H" uniqKey="Sakai H" first="H." last="Sakai">H. Sakai</name></region><name sortKey="Adachi, A" sort="Adachi, A" uniqKey="Adachi A" first="A." last="Adachi">A. Adachi</name><name sortKey="Hayami, M" sort="Hayami, M" uniqKey="Hayami M" first="M." last="Hayami">M. Hayami</name><name sortKey="Ishimoto, A" sort="Ishimoto, A" uniqKey="Ishimoto A" first="A." last="Ishimoto">A. Ishimoto</name><name sortKey="Sakuragi, J I" sort="Sakuragi, J I" uniqKey="Sakuragi J" first="J. I." last="Sakuragi">J. I. Sakuragi</name><name sortKey="Sakuragi, S" sort="Sakuragi, S" uniqKey="Sakuragi S" first="S." last="Sakuragi">S. Sakuragi</name><name sortKey="Shibata, R" sort="Shibata, R" uniqKey="Shibata R" first="R." last="Shibata">R. Shibata</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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